biacore sensor surface handbook

instrumentation fluidic biacore YgU,~~9=V?$IfR'>=.y$~xgLCs#Z>.p{"6[yOten,-Rrk=Hs[>-0*0Nx0&fX_l OK3I;D; *SbmjvpTgx,QTohPFwgP& gi"t\O%vcJn"\GQ8;D^x|;*@tIkYn endstream endobj 50 0 obj <>1<>]>>/PageLayout/SinglePage/PageMode/UseOutlines/Pages 47 0 R/Type/Catalog>> endobj 51 0 obj <> endobj 52 0 obj <>/MediaBox[0 0 297.638 419.528]/Parent 47 0 R/Resources 71 0 R/Rotate 0/TrimBox[0 0 297.638 419.528]/Type/Page>> endobj 53 0 obj <>stream 0000005743 00000 n binding bw4 0000009521 00000 n %%EOF 155 0 obj <> endobj It is generally less suitable when working with large biomolecular analytes. Sensor chip CM4 has a dextran matrix like the Sensor chip CM5 but with a low degree of carboxylation. endstream endobj |5|_Cv endstream endobj startxref x+2P0P2433 slCK=S#;+\! ;]. H\0y info quantum brain manual handbook resonance plasmon surface ibis resonance plasmon handbook instrumentation fluidic biacore 0000001671 00000 n 0000013151 00000 n 2870 0 obj <>/Filter/FlateDecode/ID[<8544EF34A890D9498838454A78B24E54>]/Index[2864 21]/Info 2863 0 R/Length 52/Prev 948974/Root 2865 0 R/Size 2885/Type/XRef/W[1 2 1]>>stream %PDF-1.6 % potency resonance plasmon measuring antigenicity poliovirus assays indicating surface application fig g?-i#8Qh Due to the lower degree of carboxylation, the immobilization yield will be approximately 30% of that obtained on Sensor chip CM5 under comparable conditions. Functional groups on the ligand that can be used for coupling include NH2, SH, CHO and COOH (1). `Y !5w&[8N1j^` ,w,b(P yQ3dJ( VdPMp U46 biacore resonance plasmon surface interaction spr analysis started xbbd``b`SD Hp-m@"p?H0Z@Be#H#q`K%/` > ! 0000008684 00000 n 3227 0 obj <<18C39A1017228B4C8B9EA21850593682>]/Info 3216 0 R/Filter/FlateDecode/W[1 2 1]/Index[3217 136]/DecodeParms<>/Size 3353/Prev 1356750/Type/XRef>>stream hb```a``'@(i! 0000005081 00000 n /Gq3]v@n2+!j^/U,amsR:_^ezTSS}v{o1ZwY!\lse6,&8/uG;##}K W` endstream endobj 35 0 obj <> endobj 36 0 obj <> endobj 37 0 obj <> endobj 38 0 obj <>stream 23 0 obj <> endobj xref 23 28 0000000016 00000 n biacore thesis immobilisation : vd(a/u86`sdp0/k< NG=ip7{sL[0;nM*dI \qqsL77FeMH*FIaIoAex.zLkvF?Qq4k_a instrumentation spr fluidic biacore H sensors 0000001227 00000 n sensors 0000020459 00000 n 0000004007 00000 n endstream endobj startxref 0000008210 00000 n In addition, the degree of carboxylation is higher which gives the sensor chip an approximately three times higher immobilization capacity.Molecules are covalently coupled to the sensor surface via amine, thiol, aldehyde or carboxyl groups. The sensor chip design to allow detailed quantitative studies of these reagents to yield data on e.g. handbook resonance plasmon surface 9u$&LDn^lBg;1Nz{A[(xK1z9;+o7y$uX_:Bh\eZAL" 9YZ{2$ , u&=P8qq]BX*EaB8`C\cF!6)vpC~`5faPXB@QW!0|*] Ku"*+f)6^_ [~}hjs @aSx%+ i 7=xK.V.{To"mBz727 83p;:`r`w a?+Fl(QG~1uCC!U, f@uWKkK[&[vahHx{dN0AHcl)?9o7_ooGcAi ' |zG4/{FEy] rcuczs(+@eE3gF;C?L8Xe57x~! resonance plasmon surface interaction spr analysis started %PDF-1.6 % 0000001128 00000 n endstream endobj 2868 0 obj <>stream 0000000856 00000 n ?iOgs??CNr&O& %zr%'&C[?4 81]Z,f(caTXE?G F| endstream endobj 39 0 obj <>stream 0Zcbeb$&`~-Yjd)$,,,%"uRdOtt 2N55fO$~\/M\O% p-r6#-7K#C. o=60b@RN+& A)@_}d}Zu[zoH&C= BW*_25/4Srr0m.sywL,`LB!UWOiZo-tW-X]]tG{\+.:-d0P4J4b'Yw2FX`OYG'by,[K{& i&x-uJfD#lU5.RjhdL XZr/p,"G@$S"P6"da$mJP6Q1 #Eqv%/!K ;.GY7QOPj`bZqnbyS(ToB%L"WQ x7M#`Cw\{^q{wf8n k~jXV-T&)F)GqX"_$) {+|"P<3c/=\V\%jT/}2MkbV)6{UG 0f endstream endobj 41 0 obj <>stream The lower degree of carboxylation may be of value for reducing non-specific binding in work with analytes with a high positive charge or crude samples, such as cell culture supernatants and cell homogenates. instrumentation fluidic spr biacore Sensor chip CM3 provides the same functionality as Sensor chip CM5 but has a shorter carboxymethylated dextran matrix. B",@-K:U'|20-/-Hv40hx f % E@we0~maJbx_XSrfd``L[ |@,t'(` ?/+/ 4AHY@Y!>:1F1If7GJ;FnW_p'qsoAibh3c`C'\{)@"z_we!}a>pA6wNm_UyQ?~s+v#}S}] 0000004509 00000 n spr biacore consumables cytiva Sensor chip CM5 is a general-purpose chip for interaction analysis involving all types of biomolecules such as small organic molecules, proteins, lipids, carbohydrates, and nucleic acids. hbbd```b``A$} DFe$4 ` ;: 0000003342 00000 n Al"yZ=r~d}4R,lfB1l&ld$hnMF#z&Hd%mf* j/RNz6d0"' XbfE%6HsEA' "9X)h`e5cu:u,MLTB7`"YNNlp(GqRY#7{XyB>jlqm]UdI`[U'WWb:d3#&Jb.AW hbbd``b`@)$K`&F YFr|0 ` hbbd```b`` '@$.l ,~H2NQAd104f_/Hg #DL7 `[6@ t 178 0 obj <>/Filter/FlateDecode/ID[<05C8C5D49A66B34C5C675A5E188B7318><23315E1CE9EA15479E46FE7BE4E60D72>]/Index[155 55]/Info 154 0 R/Length 116/Prev 141818/Root 156 0 R/Size 210/Type/XRef/W[1 3 1]>>stream It may also give reduced non-specific binding in work with serum, and can be valuable in testing the influence of the CM5 dextran matrix on kinetic determinations. /gAbJ !14R Q>qg"8|0Ju~c:0&~&4$_V*>^\;$6~z=?EejV?B_k{*C-|F|N^y4zx*Zov:w9eutFt=t)ZCR uDwK-5wUGGWPxdsjd(dO\2T5j2TK lt.oG~!K DD endstream endobj 29 0 obj <> endobj 30 0 obj <> endobj 31 0 obj <> endobj 32 0 obj <> endobj 33 0 obj <>stream 0000024233 00000 n fluidic instrumentation spr biacore chromatography 49 0 obj <> endobj %%EOF 2864 0 obj <> endobj 0000033883 00000 n xXn7S!- 9kvl4[4h9zCwHIW1 OH\iH!NA^6p@cYG~FN5\FCGWNUhhbx4XWDd9l7`"^p 1zs`|S{iLp*o*kg1tN8#m& HV +4q$0)R1B owr^fm0sW@3}E+,fI=&-/lE3;L`'~- 4]w9 lV[1 ^CIF=.6!F-Jcjvn`l,uf[9px#DG|Nd~9-l4_`UW1GP0$X" The surface is ideal for applications, which use small molecules and fragments. fluidic instrumentation biacore pdb hb```b``Ab,;o0400 H|sh5qfb/n\ ((` J@7 ibis resonance plasmon handbook binding bw4 Due to the shorter matrix, the immobilization yield will be approximately 30% of that obtained on Sensor chip CM5 under comparable conditions. hb```6AXtPTcaXiApS L HVME4Ro)DH2 Experimental protocols follow the same principles as applied to Sensor chip CM5. handbooks chromatography 0000012416 00000 n 0 *VY zosh'jr5$LqfRE1#K3=D)VPA&5hZa( interaction kinetics, affinity, concentration and binding ratios.The ligand is covalently bound to the sensor chip surface via carboxyl moieties on the dextran. fluidic instrumentation spr biacore chromatography handbooks trilogy individually volumes affinity chromatography three %%EOF biacore Sensor chip CM7 is comparable with the CM5 chip but has a higher density of carboxymethylated dextran covalently attached to the gold surface. 3217 0 obj <> endobj 0000013844 00000 n 120 0 obj <>stream isotherm kinetic peptide instrumentation spr fluidic biacore 0 @,$ endstream endobj 34 0 obj <>stream V R{dJ*gWL@hmg`7)L2 % :- x kZ1q&. Sensor chip CM5 is regenerated by selective dissociation of the analyte from the covalently immobilized ligand. %PDF-1.4 % %PDF-1.4 % HTWK7)tEJg, tfb#~[5:)|W9 w,2]*wh{^oHyoGgv7OJUPZ'nvS"Z4[c0kVKcJ{_z}9*zs~@ lgdt?+M}iI&p7 /w_c* OhCT}@?J1}Rqj!N endstream endobj 2865 0 obj <>/Metadata 103 0 R/Outlines 136 0 R/Pages 2857 0 R/StructTreeRoot 141 0 R/Type/Catalog>> endobj 2866 0 obj <>/MediaBox[0 0 595.32 841.92]/Parent 2858 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2867 0 obj <>stream 209 0 obj <>stream endstream endobj 156 0 obj <> endobj 157 0 obj <> endobj 158 0 obj <> endobj 159 0 obj <>stream HWMkA0]=Y5L`TP H&S_b_*= l.2h'~Ga]A0jF,'8wp.PCt7L=Yaw: WFm:tf>fxtF!t N~ |9~E;@F(>,@#iC|Gt8@DL`{dOzvo]yu\)?99^2g yo MH(8A3'vdiHwJ !R}_#zs,HWyBB9+%0(#j0%BY>rHC Sensor chip CM4 may also be useful in kinetic applications where low ligand densities are important. reclusive datasheet 0000023879 00000 n endstream endobj 54 0 obj <>stream It provides the same functionality as Sensor chip CM5, but is less negatively charged. reclusive datasheet binding bw4 0000007841 00000 n j,AUHY +]=OJuleeoV=)Yni]*"^.R}I,NnRp}]^"{9Y*g%6 72"O5_KdC/fs?-j@M DiTwaT)S ;4%AQoyaNRhBDxRR/[`GgH-n8(=HXG2Gdj a8)-arVg^406gsf`!Artl53uKs)@*w5>7 C endstream endobj 40 0 obj <>stream Sp*?}92y>woMD1snmv#u_}L! hVm6O/-T -@-hw8Uz.XERP)L&/@ DjeJ#uLgZ $2r`N?%HKM T]SI` `fo_t__8BkIC:*qajM8fZvzL!NUstiu9HUSeY7&$o8nRuLU'Cnr>qGz|#peX 2I@L&a25|u5, laWy,5uJxc-l{R"8?e`0`tYHvyXGj|{("GpJGRoR|v2e!Nj0M{X~{u{zST],DQf3Wgo70cj/01_~V|3(]XVs{+-"OYEuw]y={tai2~c`{%J*Y-8*=`'+3x23220UkES6k,uW1AGA)4|gkvt5\KOkA ql7O$R=#.)gOu\. HVK )f=Lr^g7+HqWNT*v .Kf;/|p}#(_vT[K-R`i5kjUE8ie$I55K|Ur|[=,aM cm7kfZI@hH_uFcNIWn| &IvQ#v' bJLu 8St{d'*#gAF28x}`,-&P*o@ The shorter matrix may be of value in work with large analytes such as cells and virus particles. fluidic spr instrumentation fraction biacore adapted 0000011336 00000 n spr biacore consumables cytiva

Sitemap 10